Temperature-Sensitive Mutations Made Easy: Generating Conditional Mutations by Using Temperature-Sensitive Inteins That Function Within Different Temperature Ranges

Temperature-sensitive mutations made easy: generating conditional mutations by using temperature-sensitive inteins that function within different temperature ranges.

Reversible and easy to use, temperature-sensitive (TS) mutations are powerful tools for studying gene function. However, TS alleles are rare and difficult to generate and identify, and this has limited their use in most multicellular organisms. We have generated and characterized 41 intein switches, temperature-sensitive Sce VMA mutations that splice only at the permissive temperatures to gener...

Temperature-sensitive mutations made easy ! Generating conditional mutations by using TS-inteins that function within different temperature ranges

Temperature-sensitive cell mutations that inhibit adenovirus 2 replication.

Five temperature-sensitive growth mutants of the hamster cell line BHK-21 were tested for the ability to support adenovirus 2 multiplication at 39 degrees and 33 degrees. Wild-type BHK-21 and mutants ts 422E and ts BCH yielded comparable amounts of virus at 33 degrees and 39 degrees, whereas in three other mutants, ts T22, ts T23, and ts AF8, virus production at 39 degrees was reduced to about ...

Temperature-sensitive mutations that arrest Arabidopsis shoot development.

To identify genes involved in meristem function we have designed a screen for temperature-sensitive mutations that cause a conditional arrest of early shoot development in Arabidopsis. We describe the characterization of three mutations, arrested development (add) 1, 2 and 3. At the restrictive temperature the add1 and add2 mutations disrupt apical meristem function as assayed by leaf initiatio...

Spontaneous temperature-sensitive mutations in bacteriophage T7.

Attempts to recover temperature-sensitive mutations affecting genes 13 and 14 (virion proteins) in bacteriophage T7 by analysis of amber revertants were confounded by the frequent occurrence of spontaneous temperature-sensitive mutations in other genes. These incidental temperature-sensitive mutations are physically distinct from but may be functionally related to genes 13 and 14, as shown by c...